The Department has an outstanding national and international reputation as a center of excellence for protein and cellular biochemistry.
Our lab is fully equipped to carry out basic and advanced research.
Recombinant protein expression and purification: medium-throughput growth facility. Spectroscopic studies: spectrophotometers, fluorimeters, rapid kinetics instruments (stopped-flows, T-jump). Affinity of ligand/protein complexes (thermodynamic parameters): Isothermal Titration Calorimetry (ITC200-Microcal/GE). Analysis of metabolites: mass spectrometry platforms, including the fast, high sensitive LTQ Orbitrap Discover ST (ThermoScientific) and the Agilent 6850A gas chromatograph coupled to a 5973N quadrupole mass selective detector. Mitochondrial function: High sensitivity O2 respirometer OROBOROS interfaced with NO selective electrodes. A novel platform for the analysis of metabolic parameters using the Seahorse analyser is currently being set up.
Molecular and Cell biology
Gene expression: real-time PCR with QPCR System Mx3000P (Agilent)
Cell lines: fully equipped cell biology facility, including CO2 incubators, biological hoods, fluorescence microscopes (Leica), Chemiluminometer Victor equipped with cell-plate reading (Perkin Elmer). If needed, a fluorescence confocal microscope is available at the newly built Facility for Nanotechnology and Nanosciences (SNN Lab) of the Sapienza University.
State of the Art crystallization robot (Biocrystal-Facility) allows to rapidly test thousand of crystallization condition using a small amount of protein (1-3 mg). Monthly schedulated beam-time allocation at synchrotron radiation sources – BESSY (Berlin – D); ESRF (Grenoble – FR) and ELETTRA (Trieste – IT) – for X-ray diffraction data collection.
Molecular docking and virtual screening: computing resources on the HPC cluster of 258 dual AMD Opteron quadcore processors, provided by CASPUR, an international institute based in Sapienza University. State of the art software for molecular docking, 3D-Pharmacophore searches, QSAR and other modeling tasks are at disposal of this RU: MOE 2009.10; Autodock 4.0; Molegro 2008.3.0.0; OpenEye’s suite of molecular modeling applications; LigandScout 3.0.